BALAKY, SALAH,TOFIK (2011) The Effect of Antibiotics on Toxin Gene Expression in PVL-positive Staphylococcus aureus Strains. Doctoral thesis, Durham University.
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Staphylococcus aureus is an extra-ordinarily versatile pathogen causing a wide spectrum of infections. The aims of this study are to analyze 10 clinical isolates of S. aureus from the UK by Multi Locus Sequence Typing (MLST) and determining their PVL-type variants. In addition to that, to study the effect of several antibiotics at sub inhibitory concentrations on a number of virulence factors at mRNA using quantitative PCR and protein levels using proteomic methods. Western blotting was used to study differential expression of Spa at protein levels.
Data showed that the 10 clinical isolates belong to seven clonal complexes (CCs), which are CC1, CC5, CC8, CC22, CC30, CC88, and CC121. Genetic variation within lukSF-PV gene showed that three of these isolates were belong to the same PVL type variant of CA-MRSA USA300 strain, R variant. From which, two isolates were found to belong to the same CC of USA300, CC8. The remaining 7 isolates were found to belong to H variant. Data presented here showed that the sub-MIC levels of both cell wall inhibitors reduced lukSF-PV and spa steady-state mRNA levels when cells were grown in the presence of these antibiotics for one hour. However, after 5 hrs post antibiotic addition of these two antibiotics, vancomycin remained depressed lukSF-PV and spa steady-state mRNA levels as well as at protein levels, but oxacillin increased spa and lukSF-PV mRNA levels, as well as Spa at protein levels. Protein synthesis inhibitors clindamycin and linezolid were both caused an increase of lukSF-PV mRNA levels, but they both decreased spa mRNA levels, when cultures grown in the presence of these antibiotics for one hour. However, when cultures grown with these antibiotics for 5 hrs, clindamycin remained to increase lukSF-PV and decrease spa mRNA levels and protein levels, but linezolid decreased both virulence factors at mRNA and protein levels.
The data showed in this study confirmed that growing S. aureus in the presence of oxacillin induce toxin expression and might enhance the virulence of this bacterium, therefore using these antibiotics to treat S. aureus infections may contribute to worse outcomes. These data also confirmed that linezolid and vancomycin, are both important selections of antimicrobial agents to treat serious infections caused by the bacterium.
|Item Type:||Thesis (Doctoral)|
|Award:||Doctor of Philosophy|
|Keywords:||PhD by Research.|
|Faculty and Department:||Faculty of Science > Biological and Biomedical Sciences, School of|
|Copyright:||Copyright of this thesis is held by the author|
|Deposited On:||05 Apr 2011 14:42|