An attempt to find cDNA clones for pea pod extensin and pea pod specific protein

Abstract

Three trials have been made for the hybridization reaction between the total RNA extract prepared from the pod of pea (Pisum sativum L.) and the probe prepared from a clone for a Brassica root protein homologous to carrot extensin, no sign of any hybridization could be seen; this shows that there is no similar sequence between those two. Another attempt was done to identify a pea pod specific clone from the cDNA library of pea pod by the use of the method of plus and minus screening technique. Two clones (pPP1052 & pPP954) have been selected that hybridized strongly to pea pod RNA but not that of pea leaf. One of them (pPP1052) has been successfully cloned into M13mpl8 and sequenced by Sanger method.