Said, Sohair S.S. (1996) Gene Expression in Neuroblastoma. Masters thesis, Durham University.
The aim of this project is to identify potential target genes (NTG's) which may be regulated by in the cell line SK-N-SH which has no amplification of theoncogene N-myc. N-mjc target genes were isolated using a modification of the protocol developed to identify p53 binding sites; this was done by generating a NTG library by isolating genomic DNA from SK-N-SH cells, digesting to completion with Sau3A, and mixing with nuclear extract from the same cell line. N-myc antibody was added and DNA-protein complexes immunoprecipitated to isolate DNA with N-Myc bound. DNA recovered from these precipitates was then cloned into pBluescript SK+. 400 clones were isolated, 4 of which were partially sequenced and compared to sequence data bases. The NTG clones showed substantial sequence identity to a number of potentially interesting genes, among them several implicated in tumour suppression, and the expression of cell surface markers. Their significance for neuroblastoma is discussed.
|Item Type:||Thesis (Masters)|
|Award:||Master of Science|
|Copyright:||Copyright of this thesis is held by the author|
|Deposited On:||24 Oct 2012 15:11|