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Durham e-Theses
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Turnip yellow mosaic virus and cell-free protein synthesis

J.W. Liddell, (1972) Turnip yellow mosaic virus and cell-free protein synthesis. Doctoral thesis, Durham University.



A polypeptide - synthesising cell-free system directed by Poly-U or Turnip Yellow Mosaic Virus-RNA was derived from imbibed seeds of Phaseolus aureus; this in vitro system was dependent upon exogenous tRNA. The Poly-U-directed system functioned in the presence of tRNAs from P.aureus, Vicia faba and yeast, whereas TYMV-RNA was translated only in the presence of tRNAs from P.aureus or V.faba. This translation barrier was related to the inability of the P.aureus high-speed supernatant enzyme fraction to charge various "protein" amino acids to yeast tRNA under uniform conditions. Such incompatibility did not exist in the Poly-U system where incubation conditions were constructed to favour only one amino acid, i.e. phenylalanine. The P.aureus enzyme fraction promoted esterification of valine to TYMV-RNA. More rigorous conditions were required for the translation of Poly-U in a Transfer System with yeast tRNA than with V.faba tRNA. Poly-U and TYMV-RNA competed for ribosomal binding sites. The characteristics of synthetic and natural templates in amino acid incorporation were correlated.

Item Type:Thesis (Doctoral)
Award:Doctor of Philosophy
Thesis Date:1972
Copyright:Copyright of this thesis is held by the author
Deposited On:13 Nov 2013 16:12

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