We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham e-Theses
You are in:

An investigation into the relationship
between Notch and Ero1

ROBINSON, NEIL,DANIEL,PATRICK (2012) An investigation into the relationship
between Notch and Ero1.
Masters thesis, Durham University.

PDF - Accepted Version


Ero1 is an endoplasmic reticulum (ER) resident protein responsible for PDI oxidation in disulphide bond formation. Recent work has shown a link between Ero1 and Notch pathway proteins. We wished to see if the oxidative folding of Notch was directly catalysed by the Ero-PDI pathway or if Ero1 was involved in transcriptional modulation of Notch signalling. Using western blot analysis, immunoprecipitation and immunofluorescence
analysis we were able to characterise and localise relevant proteins within cells. The range of cell lines and conditions under which the Ero1 antibody 2G4 were confirmed and extended.
A novel link between Ero1 and Jagged1, a notch ligand was found. In immunoprecipitation studies Ero1α was able to retrieve Jagged 1 and the opposite was also true, with Jagged 1 pulling down Ero1α as well. This suggests a novel and important link between the Ero1 and the function of
the notch signalling pathway.

Item Type:Thesis (Masters)
Award:Master of Science
Keywords:Notch, Ero1
Faculty and Department:Faculty of Science > Biological and Biomedical Sciences, School of
Thesis Date:2012
Copyright:Copyright of this thesis is held by the author
Deposited On:07 Jun 2013 12:52

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitter