Root developmental responses to heterogeneous water and nitrogen supply

Abstract

Better understanding of the interaction between the soil physical properties determining water and nitrate availability and the root proliferation and gene expression components of nutrient acquisition could contribute to food security, but may have been limited by experimental systems.
A sand rhizotron system was developed to investigate Arabidopsis (Arabidopsis thaliana) root responses to altered water and nitrate supply as manipulated by soil physical properties. When this system was compared to agar, root disparities were explained by differences in hydraulic properties, highlighting the importance of the soil physical component. The sand rhizotron system was adopted to quantify root proliferation and gene expression responses to altered water and nitrate availability in wild-type and selected mutant seedlings.
In the sand rhizotron system, primary root length and lateral root density were oppositely regulated by water availability, but similarly independent of nitrate supply. The expression of the nitrate transporter AtNRT2.1 and the aquaporin AtPIP2.2 was coordinated across all treatments. Their concentration-dependent hydraulic regulation was confirmed for AtNRT2.1 by in situ imaging of a Green Fluorescent Protein reporter line. AtNAR2.1 and AtNRT2.1 expression demonstrated independent responses to water and nitrate availability despite the requirement of AtNAR2.1 for AtNRT2.1 uptake function. Root proliferation responses to water availability under high (10.0 mM) nitrate were lost in the atnar2.1 mutant and coincided with altered hormone-associated gene (AtEIN2, AtABI4 and AtIPT5) expression. Root proliferation and AtNAR2.1 responses to water availability under high (10.0 mM) nitrate required AtPIP2.2. The coordination of root proliferation and gene expression responses to altered water and nitrate availability is proposed, that includes novel roles for AtNRT2.1, AtNAR2.1 and AtPIP2.2.