Talbot, Steven (1986) Studies on the effect of oestrogen treatment on the lipid metabolism of the male chick (gallus domesticus). Doctoral thesis, Durham University.
The aim of this study was to investigate the early (<48 h) changes in lipid metabolism occuring in the liver after 17B-oestradiol treatment (0.75 mg/100 g body wt.) of the male chick in vivo. An increase in liver weight was observed from 13 - 61 h after oestrogen treatment while an oestrogen-induced increase in the total hepatic DMA content was observed from 37 - 61 h suggesting that the early ( <37 h) liver growth response involved cell hypertrophy where as cell hyperplasia was involved in the later ( <37 h) phase of the response. Oestrogen-induced increases in total liver triacylglycerol and phospholipid were observed from 7 h post-injection while an oestrogen-induced increase in total liver free fatty acid was not observed until after 26 hours. Similarly, total triacylglycerol and phospholipid concentrations in the plasma increased progressively from 7 - 44 h after hormone treatment. Plasma levels of VLDL-triacylglycerol and VLDL-phospho- lipid in similarly-treated birds increased from 13 h and 6 h post- injection, respectively, involving an oestrogen-induced increase in the number of VLDL particles specifically enriched with phospholipid. An oestrogen-induced increase in total free fatty acids in the plasma was not observed at any time post-injection. Analysis of the fatty acid compositions of liver triacylglycerol, VLDL-triacylglycerol and VLDL-phopsholipid, accumulating in oestrogen- treated chicks, revealed that the major oestrogen-induced change was an increase in the level of oleic acid, from 13 hours post-injection. An oestrogen-induced increase in the hepatic activity of the key lipogenic enzyme acetyl-CoA carboxylase (E.C.188.8.131.52) was observed from 24 - 48 h post-injection, involving increased specific activity both with respect to protein and DNA, and total organ activity. The results from the study were correlated in an attempt to determine the early sequence (< 48 h) of oestrogen-induced changes in lipid metabolism.
|Item Type:||Thesis (Doctoral)|
|Award:||Doctor of Philosophy|
|Copyright:||Copyright of this thesis is held by the author|
|Deposited On:||08 Feb 2013 13:49|