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Durham e-Theses
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Expression of mutated pea vicilins by yeast

Elvin, Peter (1989) Expression of mutated pea vicilins by yeast. Masters thesis, Durham University.



Site directed mutagenesis has been used to introduce two cysteine residues into two hydrophilic regions of the pea vicilin polypeptide previously expressed in Saccharomyces cerevisiae. The mutated polypeptide has been expressed. The resultant protein has been characterised by SDS-Polyacrylamide gel electrophoresis (PAGE) and the presence of both inter- and intra-polypeptide sulphydryl bonds is indicated by bands of different mobility from those of the native protein. The association of mutated vicilin polypeptides into disulphide-bonded aggregates under non-denaturing conditions is not a random process, trimers being the major species produced.

Item Type:Thesis (Masters)
Award:Master of Science
Thesis Date:1989
Copyright:Copyright of this thesis is held by the author
Deposited On:08 Feb 2013 13:44

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