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Durham e-Theses
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The synthesis and processing of pea legumin in saccharomyces cerevisiae

Chieng, Hock Ming (1987) The synthesis and processing of pea legumin in saccharomyces cerevisiae. Masters thesis, Durham University.



An expression vector utilising the promoter from the yeast phosphoglycerate kinase gene and a complete protein-encoding sequence, was used to direct the expression of the pea seed storage protein, legumin, in yeast (Sciccharomyces cerevisicie) . Levels of expression of between 1.95 and 2.24% total cellular protein were obtained. The legumin polypeptide expressed in yeast has an estimated M(_r). of 59,000. In plants, the legumin precursor polypeptide is processed by co-translational proteolysis to remove a "leader" sequence and by post-translational proteolysis to generate the mature disulphide-1inked x + β polypeptides (approximate M-.'s of 38,000 and 21,000 respectively).Although "leader" sequence removal in yeast may take place, post-translational proteolysis to generate the two smaller sub-units does not occur.

Item Type:Thesis (Masters)
Award:Master of Science
Thesis Date:1987
Copyright:Copyright of this thesis is held by the author
Deposited On:08 Feb 2013 13:44

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