We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham e-Theses
You are in:

Surface phosphatase activities on roots of Pakistani wheat cultivars

Chandio, G. Mustafa (1994) Surface phosphatase activities on roots of Pakistani wheat cultivars. Masters thesis, Durham University.



A study of the surface phosphatase activity of different wheat cuhivar roots was carried out, using two substrates, p-nitrophenyl phosphate (pNPP) and 4- methylumbellferyl phosphate (4-MUP), and different pH values ranging from 3 to 10.0. Seedlings of the wheat cultivars, Indus-66, Jauhar-78 and Pavan, were grown in Hoagland solution under P-sufficient and P-limited conditions, each for 5, 10 and 15-days. The optimum pH for phosphatase activity differed in the different cultivars, but was always acidic, ranging from pH 4.0 to 6.0.P-limited plants showed greater phosphatase activity than P-suflficient ones with both substrates. Phosphatase activity increased within the first 5-day interval of the plants being deprived of phosphorus, and reached a peak by the 10-day age. In 5-day old plants, cv Pavan showed highest PMEase activity (0.06 µmol mg d.wt(^-1) h(^-1) at pH 5.0); however in cv Indus-66 (0.08 µmol mg d. wt (^-1) h(^-1)) and cv Jauhar-78 (0.07 µmol mg d. wt(^-1) h(^-1)) the highest activity was in 10-day old plants at pH 4.5 and 6.0, respectively. This occurred with both substrates. The pH optima of P-sufficient and P- limited plants were similar. As root surface phosphatase activity is an adaptive response, this could be used to distinguish cultivars suitable for growing in low inorganic phosphate fertility situations.

Item Type:Thesis (Masters)
Award:Master of Science
Thesis Date:1994
Copyright:Copyright of this thesis is held by the author
Deposited On:24 Oct 2012 15:13

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitter