We use cookies to ensure that we give you the best experience on our website. By continuing to browse this repository, you give consent for essential cookies to be used. You can read more about our Privacy and Cookie Policy.

Durham e-Theses
You are in:

Biochemical analysis of bacteriophage Orf family recombinases

WALLACE, IAIN,DAVID (2012) Biochemical analysis of bacteriophage Orf family recombinases. Masters thesis, Durham University.

PDF - Accepted Version


Bacterial pathogen evolution is an important field of study in light of the emergence of new diseases and drug resistant strains. Genomic rearrangements facilitated by temperate bacteriophages provide a major route for bacteria to acquire new pathogenic traits and disseminate them across species barriers. In phage λ, the Red pathway comprising the exo, bet and gam genes, promotes recombination in Escherichia coli at elevated frequency and with only limited sequence homology leading to numerous illegitimate exchanges. λ encodes another recombinase, Orf, which appears to supply similar functions to that of the host RecFOR proteins but has been considerably less well studied. This thesis describes the characterisation of mutations in Orf located within a proposed central DNA binding channel and a RecA interaction module. The mutants were found to impact upon single-stranded DNA binding by Orf and, in some cases, affect homodimerisation. Similar characterisation of Orf homologues from phage φETA from Staphylococcus aureus (ETA20) and E. coli K-12 cryptic prophage DLP12 (Orf151) was undertaken. In addition, another  DNA binding protein (NinH) has been subjected to bioinformatic analysis, uncovering a relationship with helix-turn-helix proteins involved in site-specific recombination and gene regulation.

Item Type:Thesis (Masters)
Award:Master of Science
Keywords:bacteriophage lambda, Orf, DNA binding, homologous recombination, NinH
Faculty and Department:Faculty of Science > Biological and Biomedical Sciences, School of
Thesis Date:2012
Copyright:Copyright of this thesis is held by the author
Deposited On:16 Aug 2012 10:42

Social bookmarking: del.icio.usConnoteaBibSonomyCiteULikeFacebookTwitter