Incorporation of pigment producing cells into novel 3D skin equivalents to study the role of the dermal compartment upon pigmentation in skin equivalents

Abstract

Recent generations of skin equivalents have started to become more complex and dynamic, which more closely capitulate the intricate workings of human skin. The importance of this research, since moving away from animal models, should not be understated. It is imperative to mimic the structure and hence the function of skin to ensure chemicals that are tested on this model system yield accurate results. As these models become more complex with inclusion of pigmentation, neurons or hair follicles, it is important to know how different models interact. Therefore, this thesis aims to understand the role of the dermal compartment and how it may influence the viability and efficacy of melanocytes within skin equivalents. The results showed that there is an important role for the dermis in providing support to the epidermis for the production of melanin and the transfer to HEKn cells in the Stratum Basale. Furthermore, there is proof of concept for the protocol and technology used to produce this pigmented skin equivalent.